CRISPRing into the woods.
Identifieur interne : 001E69 ( Main/Exploration ); précédent : 001E68; suivant : 001E70CRISPRing into the woods.
Auteurs : Chung-Jui Tsai [États-Unis] ; Liang-Jiao Xue [États-Unis]Source :
- GM crops & food [ 2164-5701 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Populus.
- méthodes : Génie génétique.
- Génome végétal, Hétérozygote, Polymorphisme de nucléotide simple, Systèmes CRISPR-Cas, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- MESH :
- genetics : Populus.
- methods : Genetic Engineering.
- CRISPR-Cas Systems, Genome, Plant, Heterozygote, Plants, Genetically Modified, Polymorphism, Single Nucleotide.
Abstract
The CRISPR/Cas9 technology is a welcome breakthrough for genome editing, owing to its precision, efficiency, versatility and ease of adoption. We recently reported the first application of CRISPR/Cas9 for biallelic mutations in stably transformed Populus, extending the species range of this powerful technology to woody perennials. An underappreciated obstacle in genome editing of outcrossing species is the frequent occurrence of sequence polymorphisms that can render CRISPR/Cas9 unproductive. We discuss experimental evidence as well as genome-wide computational analysis to demonstrate the sensitivity of CRISPR/Cas9 to allelic heterozygosity, and highlight tools and strategies that can help deal with such sequence polymorphisms. With its specificity, CRISPR/Cas9 offers a less equivocal means than previous approaches for discerning functional redundancy of paralogous genes that are prevalent in plant genomes. Continuing improvements of the CRISPR/Cas9 system for multiplex genome engineering should facilitate these efforts. The paradigm shift brought about by CRISPR/Cas9 promises to accelerate not only basic research but also applied crop improvement progress.
DOI: 10.1080/21645698.2015.1091553
PubMed: 26357840
PubMed Central: PMC5033219
Affiliations:
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University of Georgia ; Athens , GA USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>a Warnell School of Forestry and Natural Resources, and Department of Genetics; University of Georgia ; Athens </wicri:cityArea></affiliation></author><author><name sortKey="Xue, Liang Jiao" sort="Xue, Liang Jiao" uniqKey="Xue L" first="Liang-Jiao" last="Xue">Liang-Jiao Xue</name><affiliation wicri:level="2"><nlm:affiliation>a Warnell School of Forestry and Natural Resources, and Department of Genetics; University of Georgia ; Athens , GA USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>a Warnell School of Forestry and Natural Resources, and Department of Genetics; University of Georgia ; Athens </wicri:cityArea></affiliation></author></analytic><series><title level="j">GM crops & food</title><idno type="eISSN">2164-5701</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>CRISPR-Cas Systems (MeSH)</term><term>Genetic Engineering (methods)</term><term>Genome, Plant (MeSH)</term><term>Heterozygote (MeSH)</term><term>Plants, Genetically Modified (MeSH)</term><term>Polymorphism, Single Nucleotide (MeSH)</term><term>Populus (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Génie génétique (méthodes)</term><term>Génome végétal (MeSH)</term><term>Hétérozygote (MeSH)</term><term>Polymorphisme de nucléotide simple (MeSH)</term><term>Populus (génétique)</term><term>Systèmes CRISPR-Cas (MeSH)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Genetic Engineering</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Génie génétique</term></keywords><keywords scheme="MESH" xml:lang="en"><term>CRISPR-Cas Systems</term><term>Genome, Plant</term><term>Heterozygote</term><term>Plants, Genetically Modified</term><term>Polymorphism, Single Nucleotide</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Génome végétal</term><term>Hétérozygote</term><term>Polymorphisme de nucléotide simple</term><term>Systèmes CRISPR-Cas</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The CRISPR/Cas9 technology is a welcome breakthrough for genome editing, owing to its precision, efficiency, versatility and ease of adoption. We recently reported the first application of CRISPR/Cas9 for biallelic mutations in stably transformed Populus, extending the species range of this powerful technology to woody perennials. An underappreciated obstacle in genome editing of outcrossing species is the frequent occurrence of sequence polymorphisms that can render CRISPR/Cas9 unproductive. We discuss experimental evidence as well as genome-wide computational analysis to demonstrate the sensitivity of CRISPR/Cas9 to allelic heterozygosity, and highlight tools and strategies that can help deal with such sequence polymorphisms. With its specificity, CRISPR/Cas9 offers a less equivocal means than previous approaches for discerning functional redundancy of paralogous genes that are prevalent in plant genomes. Continuing improvements of the CRISPR/Cas9 system for multiplex genome engineering should facilitate these efforts. The paradigm shift brought about by CRISPR/Cas9 promises to accelerate not only basic research but also applied crop improvement progress. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26357840</PMID><DateCompleted><Year>2016</Year><Month>11</Month><Day>04</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">2164-5701</ISSN><JournalIssue CitedMedium="Internet"><Volume>6</Volume><Issue>4</Issue><PubDate><Year>2015</Year></PubDate></JournalIssue><Title>GM crops & food</Title><ISOAbbreviation>GM Crops Food</ISOAbbreviation></Journal><ArticleTitle>CRISPRing into the woods.</ArticleTitle><Pagination><MedlinePgn>206-15</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1080/21645698.2015.1091553</ELocationID><Abstract><AbstractText>The CRISPR/Cas9 technology is a welcome breakthrough for genome editing, owing to its precision, efficiency, versatility and ease of adoption. We recently reported the first application of CRISPR/Cas9 for biallelic mutations in stably transformed Populus, extending the species range of this powerful technology to woody perennials. An underappreciated obstacle in genome editing of outcrossing species is the frequent occurrence of sequence polymorphisms that can render CRISPR/Cas9 unproductive. We discuss experimental evidence as well as genome-wide computational analysis to demonstrate the sensitivity of CRISPR/Cas9 to allelic heterozygosity, and highlight tools and strategies that can help deal with such sequence polymorphisms. With its specificity, CRISPR/Cas9 offers a less equivocal means than previous approaches for discerning functional redundancy of paralogous genes that are prevalent in plant genomes. Continuing improvements of the CRISPR/Cas9 system for multiplex genome engineering should facilitate these efforts. 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